Cancer 3D Model Development

The development of cancer 3D models is to simulate the microenvironmental features of in vivo tumor growth along with the phenotypic and cellular heterogeneity of tumors. As in vivo tumors grow, 3D models more accurately mimic the complexity and variety of clinical malignancies than do 2D cell cultures. Numerous in vivo tumor features, including cell-cell communication, hypoxia, drug penetration, response, and resistance, as well as extracellular matrix synthesis and deposition, have been found to exist in 3D cell cultures. Tumor organoids have a high degree of resemblance to the original patient tumor in terms of form and mutational profile, according to histological study and DNA sequencing. These characteristics have led to the investigation of cancer 3D models as useful instruments for cancer research.

Large-scale drug screening is made possible by the ability of tumor organoids produced from numerous individuals to create extensive and well-characterized biobanks that include every molecular subtype known to exist for each type of tumor. Moreover, tumor organoids produced from patients are useful for comparing treatment sensitivity to the responses of specific patients. Furthermore, patient-derived tumor tissue may be used to determine a patient's specific course of treatment in the event that standard options are not available.

3D Model of Cancer Types
With extensive experience in 3D cell cultures, we are able to provide two well-known 3D tumor models that are obtained from patients: organoids and spheroids. These models are based on both scaffold-based and scaffold-free culture techniques.

Organoids of Tumor
The most common method for growing tumor organoids is a scaffold-based setup. They usually originate from a single adult stem cell, induced pluripotent stem cells (iPSCs), or an embryonic stem cell. Tumor organoids generated from patients more nearly mimic the intricacies of the original cancer tissue, making them an effective tool for drug development.

Spheroids of tumors
Tumor cells alone or in combination with other cell types are used to create tumor spheroids, also referred to as tumor spheres. They are used for high-throughput drug screening, particularly for medicines that target CSCs specifically, and for assessing traits associated to cancer stem cells (CSCs).