About Me
Compstatin is a 13-residue peptide that has an IC50 of 28 nM for inhibiting C3 convertase and 12 μM for inhibiting the activation of the classical and alternative complement pathways.
Compstatin has made tremendous progress in our understanding of complement inhibition.
In our investigations, we employed it to prevent complement activation and saw a noteworthy decrease in inflammatory indicators. The chemical is simple to work with and quite specific. It is a dependable tool for our lab because the outcomes were constant across several studies.
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A recombination enzyme called Cre (Cyclization Recombination Enzyme) was identified in 1981 from the P1 phage. The gene coding region is a 38 kDa polypeptide monomer protein made up of 343 amino acids, with a total length of 1029 bp. Recombination between particular locations in DNA molecules can be catalyzed by catalytically active regions found in the C-terminal domain of Cre recombinase.
The acronym for Locus of X-overP1 is LoxP. The 34 bp sequence may be found in the P1 phage and is made up of an 8 bp intermediate spacer region and two 13 bp reverse palindrome sequences. The spacer sequence controls the orientation of the LoxP sequence, whereas the reverse palindrome sequence serves as Cre recombinase's recognition and binding area.
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Ribosomes, which are essential for protein synthesis in cells, are made up of proteins and ribosomal RNA molecules. Proteins that are actively translated in the cell can be identified with the aid of ribosome complex profiling, which offers details on all ribosomes that are active in the cell at a given time. mRNA translation is a crucial process in living things that both specifies the proteome and symbolizes the flow of genetic information. The translation process is a crucial stage in the expression of genes and is involved in many physiological and developmental processes. Clarifying the mechanism and regulation of translation initiation (TI) is crucial for a deeper understanding of translational regulation.
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Because of their high sensitivity, high throughput, and low cost, microfluidic chips have steadily emerged as potent instruments for biological study in the domains of basic biology, therapeutic applications, food safety, and environmental monitoring. Among these, oligonucleotides, oligopeptides, oligosaccharides, microfluidic vitamins, minerals, and metabolites are examples of physiologically active small molecules that must be promptly detected and analyzed in physiological processes in order to guide therapeutic medicine and forecast illness prognosis. The body's tissues, organs, bones, tears, saliva, perspiration, urine, and blood all often include small biomolecules. Furthermore, bodily fluids make excellent specimens for sampling since their collection can reduce intrusion into the human body. Because biological fluids range in composition, they might affect tests differently.
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Fc-Gamma receptor assay development
FcγR binding assays for different IgG antibody isotypes or Fc fusion proteins are carried out by Creative Biolabs using Biacore T200 and 4000 systems. This system has outstanding sensitivity and is universally label-free. In order to suit your objectives, our team of scientific specialists will collaborate with you to enhance the FcγR binding assay's performance and use this knowledge to new antibody therapeutics. We can execute tests in a range of formats, including:
FcγR1/CD64
CD32a R variant and FcγR2a
CD32a H variant / FcγR2a
FcγR2b/CD32b
CD16a V variant / FcγR3a
CD16a F variant / FcγR3a
FcγR3b/CD16b
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Measuring the size distribution and concentration of exosomes is crucial for understanding their cellular functions and uptake. Recently, Tunable Resistive Pulse Sensing (TRPS) technology has become increasingly popular for characterizing exosome samples. TRPS as a powerful nanoparticle characterization system enables high-throughput, single-particle measurements by driving colloids and/or biomolecular analytes through a size-tunable nanopore, one particle at a time.
This technique is based on the principle of resistive pulse-sensing, which monitors current flow through an aperture. The tunable nanopore technology allows the regulation of ionic current and particle passage by adjusting the pore size. As each particle passes through the pore, it causes a transient change in the ionic current flow, known as a blockade event, with the amplitude of this event being termed the blockade magnitude. The blockade magnitude is proportional to the particle size, allowing accurate particle sizing after calibration with a known standard.
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Full Name:
Angiotensin I Converting Enzyme 2 (ACE2)
Function:
ACE2 is an essential counter-regulatory carboxypeptidase within the renin-angiotensin hormone system, playing a crucial role in regulating blood volume, systemic vascular resistance, and cardiovascular homeostasis. It catalyzes the conversion of angiotensin I into angiotensin 1-9, a nine-amino acid peptide with anti-hypertrophic effects on cardiomyocytes, and angiotensin II into angiotensin 1-7, which acts as a vasodilator and anti-proliferative agent, counteracting the effects of the vasoconstrictor angiotensin II. Additionally, ACE2 removes the C-terminal residue from other vasoactive peptides, including neurotensin, kinetensin, and des-Arg bradykinin, but is inactive on bradykinin. It also efficiently cleaves other biological peptides, such as apelins (apelin-13, [Pyr1]apelin-13, apelin-17, apelin-36), casomorphins (beta-casomorphin-7, neocasomorphin), and dynorphin A.
The C-terminus of ACE2 shares homology with collectrin and is involved in trafficking the neutral amino acid transporter SL6A19 to the plasma membrane of gut epithelial cells, regulating its surface expression and catalytic activity.
Check our ACE2 antibody flow cytometry
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Services at Single Cell
Utilizing our advanced spatial scRNA-seq platform, Single Cell offers a comprehensive suite of scRNA-seq sequencing and analysis services to characterize spatial gene expression changes across various tissues and cells. These services enable us to identify potential genes involved in viral entry and determine which cell types might be more susceptible to SARS-CoV-2 infection. Additionally, we integrate spatial information with differential expression detection to identify linear or periodic expression patterns, capturing multiple dimensions of infection and immune response. This includes cellular phenotypes, adaptive immune repertoires, antigen specificity, and the spatial resolution of immune tissue infiltration, providing insight into the fundamental molecular and cellular bases of complex pathophysiological signatures.
For SARS-CoV-2 research, we have applied single-cell transcriptome analyses to study the spatial expression patterns of ACE2 in various tissues and cell types from COVID-19+ patients. This approach has provided a clear understanding of the virus's entry mechanism. By combining scRNA-seq with single-molecule fluorescence in-situ hybridization (smFISH), we can observe global gene expression patterns in three dimensions. Our findings indicate that the virus's transmissivity depends on the accessibility of the ACE2 receptor, which varies based on its spatial distribution along the respiratory tract.
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With extensive experience and a team of PEGylation technical specialists, Creative Biolabs offers top-quality carbohydrate PEGylation services. We are skilled in utilizing a variety of advanced technologies to address diverse challenges. Whatever your research requirements, we are confident in developing a suitable plan for you.
Introduction to PEGylation
PEGylation offers the advantage of retaining and enhancing the desirable properties of protein therapeutics without compromising their function. The chemical, molecular, and structural properties of polyethylene glycol (PEG), along with their conformational behavior in aqueous solutions, influence the pharmacological disposition of PEGs and PEGylated products in physiological compartments. PEG molecules can vary in size and shape, providing options to modulate pharmacological outcomes for different drug therapies. PEGylating glycan structures attached to proteins allows for targeted modification at glycosylated sites, providing the benefits of PEGylation without the loss of activity that can occur with random multistep PEGylation processes.
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The transcriptome comprises the entire set of RNA transcripts present in a cell at a specific developmental stage or under particular physiological conditions. RNA, a polymeric molecule, plays crucial roles in various biological processes, including gene coding, decoding, regulation, and expression. RNA molecules encompass messenger RNA (mRNA), long non-coding RNA (lncRNA), circular RNA (circRNA), microRNA (miRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA).
Transcriptome Sequencing for Oncology
RNA sequencing (RNA-seq) is a vital tool for interpreting the functional elements of the genome and understanding the mechanisms underlying various diseases, particularly cancers. While microarray-based approaches have been used for large-scale, high-throughput RNA studies, such as identifying differentially expressed genes, they are limited by their hybridization-based nature. The advent of massively parallel DNA sequencing technologies has enabled RNA-seq by sequencing complementary DNA (cDNA). This method offers superior resolution and higher reproducibility compared to traditional microarray technologies. RNA-seq allows for a deeper understanding of alternative splicing events, novel genes and transcripts, and fusion transcripts. It also aids in elucidating molecular mechanisms and signaling pathways that control embryonic development. Transcriptome data provide a valuable starting point for researchers investigating the functions of newly discovered genes.
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