Services at Single Cell

Services at Single Cell
 

Utilizing our advanced spatial scRNA-seq platform, Single Cell offers a comprehensive suite of scRNA-seq sequencing and analysis services to characterize spatial gene expression changes across various tissues and cells. These services enable us to identify potential genes involved in viral entry and determine which cell types might be more susceptible to SARS-CoV-2 infection. Additionally, we integrate spatial information with differential expression detection to identify linear or periodic expression patterns, capturing multiple dimensions of infection and immune response. This includes cellular phenotypes, adaptive immune repertoires, antigen specificity, and the spatial resolution of immune tissue infiltration, providing insight into the fundamental molecular and cellular bases of complex pathophysiological signatures.
 

For SARS-CoV-2 research, we have applied single-cell transcriptome analyses to study the spatial expression patterns of ACE2 in various tissues and cell types from COVID-19+ patients. This approach has provided a clear understanding of the virus's entry mechanism. By combining scRNA-seq with single-molecule fluorescence in-situ hybridization (smFISH), we can observe global gene expression patterns in three dimensions. Our findings indicate that the virus's transmissivity depends on the accessibility of the ACE2 receptor, which varies based on its spatial distribution along the respiratory tract.