TRPS-based Exosome Characterization

Measuring the size distribution and concentration of exosomes is crucial for understanding their cellular functions and uptake. Recently, Tunable Resistive Pulse Sensing (TRPS) technology has become increasingly popular for characterizing exosome samples. TRPS as a powerful nanoparticle characterization system enables high-throughput, single-particle measurements by driving colloids and/or biomolecular analytes through a size-tunable nanopore, one particle at a time.
 

This technique is based on the principle of resistive pulse-sensing, which monitors current flow through an aperture. The tunable nanopore technology allows the regulation of ionic current and particle passage by adjusting the pore size. As each particle passes through the pore, it causes a transient change in the ionic current flow, known as a blockade event, with the amplitude of this event being termed the blockade magnitude. The blockade magnitude is proportional to the particle size, allowing accurate particle sizing after calibration with a known standard.