Transcriptome Sequencing (RNA-seq)

Gene expression techniques based on microarrays have been applied to high-throughput, large-scale RNA-level investigations, including the identification of genes with differential expression. Nevertheless, the hybridization-based nature of microarray technology has restricted their application. RNA-seq through cDNA sequencing has been made possible by developments in massively parallel DNA sequencing methods. Compared to conventional microarray technologies, RNA-seq is a highly advantageous instrument that delivers improved resolution and increased reproducibility. We can learn more about new genes and transcripts, fusion transcripts, and alternative splicing events via RNA-seq. Additionally useful for comprehending molecular mechanisms, RNA-seq provides information on the signaling pathways that govern embryonic development. The transcriptome data provide a solid starting point for investigating the function of recently discovered genes.