Secondary Antibodies

In order to help in the identification, categorization, and isolation of the target antigen, secondary antibodies attach to the primary antibody. The secondary antibody needs to be specific for the isotype and antibody species of the primary antibody used in the application in order to guarantee detection. Usually, secondary antibodies are polyclonal.

Secondary antibody conjugates are useful instruments for cell-based and biochemical examination. Because they provide signals quickly and robustly, enzyme-labeled secondary antibodies are frequently utilized in a variety of experiments. They also have high catalytic turnover rates. In applications such as immunofluorescence microscopy and cell imaging that involve protein and cell analysis, secondary antibodies coupled to fluorescent dyes provide stronger signals and multiplexing capabilities. When detecting target antigens of low abundance, high sensitivity and signal amplification are two desirable properties for both enzyme-labeled and dye-labeled secondary antibodies.

For more: isotype control antibody