Single Cell Sorting and Isolation

Sorting cells by type from sample materials is accomplished using single-cell sorting. The main criteria used to isolate them include variations in morphology (or size), expression of surface proteins, and other characteristics. The usual process involves obtaining viable, undamaged cells through enzymatic or mechanical dissociation, followed by the isolation of individual cells from the dissociated suspension. The two groups that comprise the present cell isolation technologies are based on the diversity of concepts.