Because of FcRn-mediated recycling, IgG molecules belonging to the IgG1, IgG2, and IgG4 subclasses have remarkably extended half-lives. Therapeutic antibodies have been reported to have half-lives as long as four weeks. The CH2 and CH3 domains combine to produce the binding site for FcRn, which is located in the Fc region. Therefore, fusing to an Fcγ region confers on a therapeutic protein the immunoglobulin half-life extension features (larger size, FcRn recycling). Most fusion proteins have be...

  Single Cell Therapeutics Single-cell solutions can be used, based on the SARS-CoV-2 infection, to investigate the potential tropism of SARS-CoV-2, characterize phenotypic diversity before, during, and after SARS-CoV-2 infection, analyze new immune cell subpopulations in health and disease, and investigate potential cell surface markers and stage-specific pathogen antigens. These variations all contribute significantly to the search for new antiviral medications and the creation of viable...

Gene expression techniques based on microarrays have been applied to high-throughput, large-scale RNA-level investigations, including the identification of genes with differential expression. Nevertheless, the hybridization-based nature of microarray technology has restricted their application. RNA-seq through cDNA sequencing has been made possible by developments in massively parallel DNA sequencing methods. Compared to conventional microarray technologies, RNA-seq is a highly advantageous inst...

The single-chain triplebody, also known as the recombinant Anti-EGFR x Anti-CD16 Bispecific Antibody, is intended to be expressed as a single chain containing three scFv units, two distal binding sites for EGFR, and a central site for CD16. At the C/N terminus, it has a His-tag for affinity purification. NK cells can be redirected to tumor cells by this BsAb. It is intended for use in studies on solid tumor therapy, non-small cell lung cancer (NSCLC), colorectal cancer (CRC), glioblastoma multif...

Numerous studies have shown that the identification and validation of plasma glycoproteins with altered glycosylation can provide insight into critical events in the progression of cancer and may even serve as potential glycoprotein biomarkers for the detection of cancers or other diseases. Despite the fact that plasma glycoprotein plays an important role in a variety of physiological activities, there is a dearth of research and literature regarding plasma glycoprotein profiling, and many obsta...

Compstatin is a 13-residue peptide that has an IC50 of 28 nM for inhibiting C3 convertase and 12 μM for inhibiting the activation of the classical and alternative complement pathways. Compstatin has made tremendous progress in our understanding of complement inhibition. In our investigations, we employed it to prevent complement activation and saw a noteworthy decrease in inflammatory indicators. The chemical is simple to work with and quite specific. It is a dependable tool for our lab be...

A recombination enzyme called Cre (Cyclization Recombination Enzyme) was identified in 1981 from the P1 phage. The gene coding region is a 38 kDa polypeptide monomer protein made up of 343 amino acids, with a total length of 1029 bp. Recombination between particular locations in DNA molecules can be catalyzed by catalytically active regions found in the C-terminal domain of Cre recombinase.  The acronym for Locus of X-overP1 is LoxP. The 34 bp sequence may be found in the P1 phage and is made...

Ribosomes, which are essential for protein synthesis in cells, are made up of proteins and ribosomal RNA molecules. Proteins that are actively translated in the cell can be identified with the aid of ribosome complex profiling, which offers details on all ribosomes that are active in the cell at a given time. mRNA translation is a crucial process in living things that both specifies the proteome and symbolizes the flow of genetic information. The translation process is a crucial stage in the exp...

Because of their high sensitivity, high throughput, and low cost, microfluidic chips have steadily emerged as potent instruments for biological study in the domains of basic biology, therapeutic applications, food safety, and environmental monitoring. Among these, oligonucleotides, oligopeptides, oligosaccharides, microfluidic vitamins, minerals, and metabolites are examples of physiologically active small molecules that must be promptly detected and analyzed in physiological processes in order ...

Fc-Gamma receptor assay development FcγR binding assays for different IgG antibody isotypes or Fc fusion proteins are carried out by Creative Biolabs using Biacore T200 and 4000 systems. This system has outstanding sensitivity and is universally label-free. In order to suit your objectives, our team of scientific specialists will collaborate with you to enhance the FcγR binding assay's performance and use this knowledge to new antibody therapeutics. We can execute tests in a range of formats,...

Measuring the size distribution and concentration of exosomes is crucial for understanding their cellular functions and uptake. Recently, Tunable Resistive Pulse Sensing (TRPS) technology has become increasingly popular for characterizing exosome samples. TRPS as a powerful nanoparticle characterization system enables high-throughput, single-particle measurements by driving colloids and/or biomolecular analytes through a size-tunable nanopore, one particle at a time.   This technique is base...

Full Name: Angiotensin I Converting Enzyme 2 (ACE2)   Function: ...