Creative Diagnostics, a leading manufacturer and supplier of antibodies, antigens and assay kits, has announced the availability of a comprehensive range of highly validated DNA/RNA methylation antibodies for researchers studying the critical role of methylation in cancer development and progression. These antibodies are designed to facilitate groundbreaking research in epigenetics and cancer diagnostics.
To date, at least 17 and 160 chemical modifications have been identified in DNA and RNA, respectively. With more than 70 of the known RNA modifications containing methyl groups, the importance of methylation modifications cannot be overstated. Analysis of nucleic acid methylation and its functional effects is of critical importance to epigenetic researchers. In addition, methylation profiles of the epigenome can be used for disease identification, research and therapeutic development.
The detection of methylation in DNA and RNA is particularly important for the diagnosis and treatment of various tumor diseases. CpG island DNA methylation plays a key role in X chromosome inactivation, imprinting, and cancer, and is rare in normal cells and increases with age. There is evidence for hypermethylation of CpG island promoters of tumor suppressor genes such as p16INK4a in melanoma, SET9 in cervical cancer, and p15INK4b in hematologic malignancies.
On the other hand, RNA methylation greatly influences tissue development, DNA damage repair, and tumorigenesis and progression by regulating RNA stability. The m6A methyltransferase METTL3 is overexpressed in gastric, colorectal, and hepatocellular carcinomas and promotes cancer cell proliferation by inhibiting the tumor suppressor SOCS2. The m5C methyltransferase NSUN2 is overexpressed in breast and colorectal malignancies.
Antibodies against nucleic acid modifications have played a critical role in epigenetics and epitranscriptomics. For example, enrichment of DNA containing 5mC by methylated DNA immunoprecipitation (MeDIP) has been widely used in epigenetics for decades. Additionally, antibody-based sequencing techniques have been widely used for transcriptome studies of a variety of RNA modifications, including m6A, m1A, m5C, and m7G.
Antibody-based methods are relatively simple, fast, and cost-effective, but rely on highly specific antibodies to recognize methylated DNA/RNA. The key to generating antibodies that effectively detect RNA modifications is antigen design and antibody validation. Creative Diagnostics now offers a number of highly validated antibodies for DNA/RNA methylation analysis, such as Rabbit Anti-5-methylcytosine (5-mC) monoclonal antibody, clone SN342, Rabbit Anti-5-methylcytosine (5-mC) monoclonal antibody, clone SN342, and Sheep Anti-5-Methylcytosine polyclonal antibody. These antibodies are rigorously validated and optimized for various immunological applications, ensuring consistent and reliable results.
The company also offers a wide selection of antibodies targeting different methylation sites, providing researchers with flexibility in their experiments. Creative Diagnostics' comprehensive portfolio of methylation antibodies are essential tools for advancing the understanding of cancer and developing novel therapeutic approaches.
Creative Diagnostics is committed to providing researchers with the products they need for epigenetics and cancer research. To learn more about these antibodies and other research tools, please visit https://www.creative-diagnostics.com/dna-rna-methylation-antibodies.htm.
About Creative Diagnostics
Creative Diagnostics is a leading manufacturer and supplier of antibodies, viral antigens, innovative diagnostic components, and critical assay reagents. In addition to providing contract R&D and biologic manufacturing services for diagnostic manufacturers along with GMP biologics manufacturing for the biopharmaceutical market, the company aims to continue to act as a trusted source for all researchers' assay development and manufacturing needs.
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As an expert in providing solutions to assist virology and microbiology research, Creative Diagnostics has announced the launch of its advanced Respiratory Syncytial Virus Animal Models for researchers and scientists worldwide focused on advancing RSV research and accelerating the development of effective vaccines and therapeutics against RSV.
Human respiratory syncytial virus (hRSV) is an enveloped, single-stranded, negative-sense RNA virus that represents a significant public health burden in both developing and developed countries. Scientists have made concerted efforts to develop various animal models to study different aspects of hRSV. However, the high specificity of hRSV for the human host has greatly hindered the development of specialized animal models. In addition, because no single animal model reflects all aspects of this viral infection and disease, a variety of models have been used to study RSV, ranging from rodents and small mammals to large animals and non-human primates (NHPs).
With the exception of chimpanzees, nonhuman primates are only partially susceptible to hRSV replication. After infection, nonhuman primates have mild or no clinical signs and generally show mild lung lesions. Cotton rats, mice, ferrets, chinchillas, guinea pigs, hamsters, and newborn lambs are also not susceptible to RSV infection. Additionally, cotton rats and mice have been invaluable in the development of monoclonal antibody prophylaxis for high-risk infants and have provided insights into RSV immunity and pathogenesis.
RSV animal models are important tools for studying the complex dynamics of RSV infection, evaluating potential therapies, and developing effective vaccines. With advanced animal modeling technology and unparalleled infectious disease expertise, Creative Diagnostics provides researchers and scientists with reliable respiratory syncytial virus animal models to facilitate the development of therapies and vaccines. Its team of experts can provide clients with the most appropriate RSV animal model based on the infection to be studied and the proposed research hypothesis.
Creative Diagnostics offers a full range of services to support RSV animal model research, including study design and execution, pathological analysis and immune response profiling, as well as in vivo pharmacokinetic studies and pharmacodynamics/efficacy/toxicity assessments. These RSV animal model services allow scientists to evaluate the immunogenicity and efficacy of potential RSV vaccine candidates in preclinical studies, investigate RSV pathogenesis to identify novel therapeutic targets, and assess the safety and efficacy of antiviral and immunomodulatory compounds.
With extensive expertise in biological research, Creative Diagnostics offers RSV Cotton Rat Models and RSV Mouse Models to advance RSV research, vaccine development, and drug discovery. By providing RSV animal models, Creative Diagnostics aims to help accelerate the development of effective treatments and improve patient outcomes. With this new offering, Creative Diagnostics reinforces its commitment to providing high-quality research tools and services to advance the understanding and treatment of infectious diseases.
For more information on the RSV animal models, please visit https://antiviral.creative-diagnostics.com/respiratory-syncytial-virus-animal-model.html.
About Creative Diagnostics
Headquartered in New York, Creative Diagnostics is a consulting and experimental service provider specializing in virology and microbiology. The company provides comprehensive solutions to conquer obstacles in virology and microbiology research, from high-security infrastructure provision, biosafety regulation elucidation, to expert viral system assistance.
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Creative Diagnostics, a leading manufacturer and supplier of antibodies, small molecule conjugates and critical assay reagents for the analysis of food, feed, environmental and toxicological samples, has introduced its comprehensive range of T-2/HT-2 Toxin Test Reagents to meet the growing demand for accurate and efficient detection of T-2 and HT-2 mycotoxins, ensuring food safety and consumer protection.
T-2 and HT-2 toxins are type A trichothecene mycotoxins produced by fungi of the genus Fusarium. Since T-2 toxin is readily metabolized to HT-2 toxin and T-2 and HT-2 toxins are often found together in infected grain, the two mycotoxins are often analyzed together. The combination of T-2 and HT-2 affects many crops, especially grains and feeds such as corn, wheat, barley, millet, corn gluten feed, corn gluten meal, corn DDGS and bran.
These mycotoxins can cause illness in animals and humans if diseased grains are consumed. Animals affected by the toxins include swine, dairy cattle, poultry, dogs, cats and horses. Effects of the toxins include digestive disorders, bleeding, edema, oral lesions, dermatitis, and blood disorders. In addition, T-2 toxin is the primary causative toxin in human gastrointestinal toxic leukemia.
As food safety awareness has increased in recent years, the European Union (EU) recommended maximum levels for both toxins in food and feed in 2013. To comply with these EU guidelines, grain must be analyzed accurately and reliably. Creative Diagnostics has developed a robust portfolio of T-2/HT-2 Toxin Test Reagents to detect the presence of T-2 and HT-2 toxins in various food products, empowering laboratory researchers, food manufacturers, and regulatory agencies to safeguard public health.
The product line includes a comprehensive array of reagents, such as T2-toxin ELISA Kits, HT2-Toxin Standard, T-2 Toxin Test, and RHA™ anti-T-2 toxin monoclonal antibody. For example, the T2-toxin ELISA Kit is designed for quantitative measurement of T-2 toxin in feed, and the T-2 Toxin Test can be used for rapid qualitative analysis of T-2 Toxin in grain/feed. These reagents offer exceptional sensitivity and specificity, enabling precise quantification of these toxins in diverse food matrices.
Creative Diagnostics is committed to developing high quality reagents to support the advancement of mycotoxin detection. Through years of research and technical staff experience, Creative Diagnostics has successfully developed a range of antigens and antibodies for use in immunoassays and has established ELISA and lateral flow immunoassay test platforms. As a result, this new product line of T-2/HT-2 Toxin Test Reagents, backed by the company’s extensive R&D, can ensure optimal performance and reliable results.
Creative Diagnostics is dedicated to supporting the global food safety community by offering comprehensive solutions for mycotoxin detection and analysis. For more information about the testing products and the company's comprehensive portfolio of food safety solutions, please visit https://www.creative-diagnostics.com/food-analysis/tag-t-2-/-ht-2-toxin-39.htm.
About Creative Diagnostics
Creative Diagnostics is a leading manufacturer and supplier of antibodies, small molecule conjugates and critical assay reagents for the analysis of food, feed, environmental and toxicological samples. Its product portfolio covers a wide range of analytes including mycotoxins, drug residues, pesticides, contaminants, food allergens, food pathogens and vitamins. The company also assists customers in the rapid development, manufacture and commercialization of small molecule antigens, antibodies and food safety lateral flow strips for any market segment.
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Creative Diagnostics, a reagent supplier and developer focused on biologics quality control, is excited to announce the launch of its Mammalian DNA Residue Assay Kits (qPCR) to the research community for precise and reliable detection and quantification of residual mammalian host cell DNA in biopharmaceutical products, ensuring stringent quality control and regulatory compliance.
Detection of residual DNA in host cells throughout the biopharmaceutical manufacturing process is an important step in ensuring product safety and batch validation. Among the methods for detecting residual DNA, qPCR is considered the most practical method due to its sensitivity, accuracy, precision and time-saving. Creative Diagnostics now offers a comprehensive range of Mammalian DNA Residue Assay Kits (qPCR) to accurately detect and quantify residual mammalian host cell DNA throughout the biopharmaceutical manufacturing process, including the Vero DNA Residue Assay Kit, MDCK DNA Residue Assay Kit, CV-1 DNA Residue Assay Kit, CHO DNA Fragment Residue Assay Kit, and BHK DNA Residue Assay Kit.
For example, the MDCK DNA Residue Assay Kit is based on the principle of the PCR fluorescent probe method for the quantification of MDCK residual DNA in samples, which is fast, specific and reliable with a minimal limit of detection for fg levels. The kit is supplied with a reference standard for the quantification of MDCK DNA and can be used in conjunction with the Creative Diagnostics Host Cell DNA Pretreatment Kit to accurately determine the amount of residual MDCK DNA in biological samples.
Another example is the HEK293 DNA Fragment Residue Assay Kit. Using the PCR fluorescent probe method, four different amplification fragments (75 bp, 122 bp, 244 bp, 562 bp) have been designed to quantitatively analyze the size distribution of residual HEK293 DNA fragments in samples. The kit is supplied with a reference standard for HEK293 DNA quantification. It can be used in combination with the Creative Diagnostics Host Cell DNA Pretreatment Kit to accurately determine the amount of residual HEK293 DNA in biological samples.
By leveraging these advanced assay kits, biopharmaceutical manufacturers can significantly improve their operations by reducing product safety risks associated with residual host cell DNA, improving product quality and consistency, ensuring compliance with stringent regulatory guidelines, and optimizing downstream purification processes. These kits represent a significant advancement in biopharmaceutical quality control, enabling professionals to produce safe and effective products that meet the highest regulatory standards.
Creative Diagnostics is dedicated to supporting the biopharmaceutical industry with comprehensive quality control solutions. The company offers a full range of products and services, including assay kits, reagents and custom services, to meet the diverse needs of researchers and manufacturers. For more information about Mammalian DNA Residue Assay Kits and other product offerings, please visit https://qbd.creative-diagnostics.com/products/mammalian-dna-residue-assay-kits-qpcr-2747.html.
About Creative Diagnostics
Creative Diagnostics is a global leader in the development and manufacturing of innovative tools and reagents for bioprocess impurity analysis. The company offers a comprehensive portfolio of solutions to support researchers in the quality control of biologics and provides biopharmaceutical quality, purity and safety assays, analytical methods and applications for the biotechnology and biopharmaceutical industries.
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Creative Diagnostics, a leading manufacturer and supplier of antibodies, antigens and assay kits, is pleased to announce the availability of its highly specific Anti-Adenovirus Monoclonal Antibody. This research tool offers exceptional accuracy and sensitivity for the detection of adenoviruses in various applications such as IF, ICC, and ELISA.
Adenoviruses (members of the family Adenoviridae) are medium-sized (90-100 nm), nonenveloped viruses with icosahedral nucleocapsids containing a double-stranded DNA genome. The name derives from the first isolation of an adenovirus from human adenoids in 1953. Adenoviruses DNA viruses are generally widespread in nature with a variety of vertebrate hosts. In humans, 88 human adenoviruses have been identified in seven populations (A through G) and more than 50 different adenovirus serotypes have been identified, causing a wide range of diseases from mild respiratory infections in young children to life-threatening multi-organ disease in people with compromised immune systems.
Culture is the gold standard for diagnosis of adenovirus infection. However, culture results can take up to 3 weeks. Serological tests have a faster turnaround time but may not be as sensitive as culture. Because of the clinical importance of rapid laboratory diagnosis, direct detection of viral antigens in nasopharyngeal secretions, feces, or both is used as the primary diagnostic test for adenovirus infection.
Immunofluorescence (IF) was the first method used for rapid diagnosis of respiratory infections caused by viruses. In the hands of an experienced microscopist, the method is both sensitive and specific. Individual specimens can be tested on arrival at the laboratory and results are available within a few hours. The most important aspect of IF is specimen collection and processing, and a sufficient number of infected respiratory epithelial cells should be obtained.
Adenoviruses are a common cause of respiratory illness, and effective research tools are crucial for understanding and developing treatments for these infections. Recognizing the importance of accurate diagnosis of adenoviruses for effective treatment and to support the adenovirus research, Creative Diagnostics now offers new Anti-Adenovirus Monoclonal Antibodies to help researchers to identify and study these viruses. In addition, Creative Diagnostics offers qualitative or quantitative ELISA kits for the detection of ADV antibodies in serum or plasma.
Creative Diagnostics provides Anti-Adenovirus Monoclonal Antibody in various formats and quantities to meet specific research needs. These highly specific antibodies target the hexon protein of adenoviruses, making them a valuable tool for researchers studying these common pathogens. Additionally, the antibody’s high specificity and affinity ensure accurate and reliable detection of adenoviruses in various research applications such as IF.
Creative Diagnostics offers a comprehensive range of high-affinity Anti-Adenovirus Monoclonal Antibodies for research and assay development. To learn more about this product and other Adenovirus research tools, please visit https://www.creative-diagnostics.com/Anti-Adenovirus-hexon-protein-MAb-FITC-Conjugated-129647-491.htm.
About Creative Diagnostics
Creative Diagnostics is a leading manufacturer and supplier of antibodies, viral antigens, innovative diagnostic components, and critical assay reagents. In addition to providing contract R&D and biologic manufacturing services for diagnostic manufacturers along with GMP biologics manufacturing for the biopharmaceutical market, the company aims to continue to act as a trusted source for all researchers' assay development and manufacturing needs.
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As an expert in providing solutions to assist virology and microbiology research, Creative Diagnostics is proud to announce the launch of its Hemagglutination Assay (HA) services for effective virus and antibody detection. The new anti-virus testing solution offers a valuable tool for researchers working on influenza virus characterization, vaccine development, and general viral diagnostics.
Certain viruses agglutinate red blood cells in certain animals, a process known as viral hemagglutination. HA test is designed to infer the presence of the virus in the material being tested and to determine whether this response is specific. It is a well-established method that is widely used in influenza vaccine, diagnostic and surveillance to measure virus and antibody titers and to monitor influenza subtypes.
Hemagglutination occurs when the hemagglutinin protein on the surface of the influenza virus binds to a serine on red blood cells (RBCs). Similarly, when sufficient numbers of virus and RBCs are present, the virus particles act as a bridging agent, forming a network that connects the RBCs, resulting in hemocoagulation.
The hemagglutination assay is the most commonly used method for detecting influenza virus, adenovirus, rabies virus, and many other microorganisms. It is the basis for a wide range of veterinary and life science diagnostic tests. HA analysis is simple, uses relatively inexpensive and commonly available instruments and consumables, and provides results within a few hours. The method has been well validated in many laboratories around the world and has a degree of reliability, comparability and standardization.
In addition, the ability of viruses to agglutinate RBCs can be inhibited by specific antibodies, leading to the development of the hemagglutination inhibition (HAI) assay. The HA-HAI test allows the identification of unknown viruses in known sera and the titration of antibodies in unknown sera with known viruses by determining the level of the corresponding antibodies. The hemagglutination assay, used in conjunction with interactions, produces a visible network showing the relative concentrations of viruses and antiviral antibodies.
Creative Diagnostics now offers Hemagglutination Assay services and customized early detection solutions to life science researchers focused on antiviral and infectious disease diagnostics. The research team combines infectious disease and analytical expertise to provide customers with the most powerful portfolio of antiviral and antimicrobial in vitro testing services.
For example, the Creative Diagnostics team uses a wide range of erythrocytes from different species (chicken, guinea pig, goose, horse, cow, sheep, rabbit, and human A, B, AB, and O) and standardizes experiments using reference standards. In addition, because the aggregation capacity of RBCs declines over time, the team prepares fresh RBCs each time to ensure reproducible results.
Creative Diagnostics offers a comprehensive portfolio of antiviral testing services, including the Hemagglutination Assay. For more information on how this assay can benefit your research, please visit https://antiviral.creative-diagnostics.com/hemagglutination-assay-ha.html.
About Creative Diagnostics
Headquartered in New York, Creative Diagnostics is a consulting and experimental service provider specializing in virology and microbiology. The company provides comprehensive solutions to conquer obstacles in virology and microbiology research, from high-security infrastructure provision, biosafety regulation elucidation, to expert viral system assistance.
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Creative Diagnostics, a leading manufacturer and supplier of antibodies, small molecule conjugates and critical assay reagents for food, feed, environmental and toxicological sample analysis, announces the availability of its high-quality Patulin Test Reagents. These reagents are suitable for assisting food producers and regulatory agencies in ensuring the safety of apples and apple-derived products.
Patulin is a secondary metabolite of certain fungi of the genera Penicillium, Aspergillus and Batrachytrium, but more specifically Penicillium expansum appears to be the fungus that produces patulin. It is a common contaminant of apples, and levels of patulin in apple products are often used as a measure of apple quality in food production. Patulin can be found in apple juice, apple sauce, apple puree, baby food and cider. It has also been found in other foods such as cereals, fruits and vegetables.
Patulin has been found to cause a variety of health problems in laboratory animals. There is concern that humans may be similarly affected and exposure should be minimized. Legal limits vary from country to country, and the World Health Organization recommends a maximum concentration of 50 µg/L in apple juice. In the European Union, the limit is set at 50 micrograms per kilogram (µg/kg) for both apple juice and cider, and at half that concentration, 25 µg/kg, for solid apple products and 10 µg/kg for products for infants and young children.
Creative Diagnostics is committed to providing high-quality testing solutions to help protect consumers from the potential health risks associated with Patulin contamination. The company now offers a comprehensive portfolio of Patulin Test Reagents for food safety applications, including Patulin ELISA Kit, Patulin Standard (95%), and Anti-Patulin Polyclonal Antibody. For example, the Patulin ELISA Kit (Catalog# DEIANJ49) is a direct competitive ELISA based on the recognition of Patulin by specific antibodies.
Creative Diagnostics Patulin Test Reagents provide food manufacturers with a reliable and accurate method for detecting Patulin in apple juice, applesauce, baby food and other apple products. By utilizing Creative Diagnostics' Patulin Test Reagents, food manufacturers can ensure their products meet the highest safety standards. For example, they can monitor the quality of their apples and make sure their products comply with food safety regulations. Most importantly, early detection of patulin contamination helps prevent contaminated products from reaching consumers.
Creative Diagnostics is dedicated to providing quality reagents for safeguarding food quality and protecting public health. These highly specific Patulin Test Reagents offer scientists a valuable and efficient tool to detect the presence of patulin. For more information about food safety testing products, please visit https://www.creative-diagnostics.com/food-analysis/tag-patulin-37.htm.
About Creative Diagnostics
Creative Diagnostics is a leading manufacturer and supplier of antibodies, small molecule conjugates and critical assay reagents for the analysis of food, feed, environmental and toxicological samples. Its product portfolio covers a wide range of analytes including mycotoxins, drug residues, pesticides, contaminants, food allergens, food pathogens and vitamins. The company also assists customers in the rapid development, manufacture and commercialization of small molecule antigens, antibodies and food safety lateral flow strips for any market segment.
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Creative Diagnostics, a leading manufacturer and supplier of antibodies, antigens and assay kits, is pleased to announce the availability of its new line of Norovirus VLP Antigens to accelerate in vitro diagnostics and vaccine research. These new antigens have been validated to a high level of confidence using electron microscopy.
Norovirus is a non-enveloped, positive-sense, single-stranded RNA virus of the family Caliciviridae and is the leading cause of non-bacterial acute gastroenteritis worldwide. The infection is characterized by non-bloody diarrhea, vomiting and abdominal pain. The stability of viral particles is of particular interest because noroviruses are transmitted by the fecal-oral route and they can persist in the environment. Studies of the assembly and degradation of norovirus capsids have relied heavily on norovirus-like particles.
The norovirus genome is 7.4-7.7 kb in size and has three open reading frames (ORFs). Nonstructural proteins, such as RNA-dependent polymerase (RdRp), are encoded by ORF1, while structural proteins are encoded by ORF2 (VP1) and ORF3 (VP2). Structural analysis of noroviruses has shown that each viral capsule consists of 90 dimers of VP1 with T = 3 icosahedral symmetry. Various expression systems have been developed to produce viral capsids in the form of virus-like particles (VLPs).
Noroviruses are classified into genomic groups and genotypes based on the amino acid diversity of the VP1 protein. According to this system, the genus Norovirus is divided into 10 genomes (GI-GX) and 49 genotypes (9 GI, 27 GII, 3 GIII, 2 GIV, 2 GV, 2 GVI, and 1 each of GVII, GVIII, GIX [formerly GII.15 ], and GX), of which the genomes known to be infectious to humans include GI, GII, GVIII, and GIX. GII.4 is of particular importance as it has caused numerous outbreaks worldwide over the years and is believed to be the primary cause of human infections.
Creative Diagnostics now offers a series of yeast-expressed Norovirus VLP antigens for in vitro diagnostic and vaccine studies, including Recombinant Norovirus GI.1 VLP, Recombinant Norovirus GII.3 VLP, and Recombinant Norovirus GII.17 VLP. These antigens have been validated with high confidence by electron microscopy and provide researchers and diagnosticians with a powerful tool for studying and detecting Norovirus infections. Creative Diagnostics' team of experts is also available to assist researchers in selecting the most suitable antigens for their specific studies.
The purified Norovirus VLP Antigens are an example of Creative Diagnostics' commitment to providing innovative and reliable products to the research and diagnostic communities. Its team has worked diligently to develop antigens that are not only highly specific but also offer excellent sensitivity, making them ideal for a wide range of diagnostic and research applications.
Creative Diagnostics offers a comprehensive range of Norovirus VLP Antigens catering to diverse research needs. For more information on the new antigens, please visit https://www.creative-diagnostics.com/new-norovirus-vlp-antigens.htm.
About Creative Diagnostics
Creative Diagnostics is a leading manufacturer and supplier of antibodies, viral antigens, innovative diagnostic components, and critical assay reagents. In addition to providing contract R&D and biologic manufacturing services for diagnostic manufacturers along with GMP biologics manufacturing for the biopharmaceutical market, the company aims to continue to act as a trusted source for all researchers' assay development and manufacturing needs.
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As an expert in providing solutions to assist virology and microbiology research, Creative Diagnostics is proud to announce the launch of its comprehensive suite of Plaque Reduction Assay services to support antiviral research. These new solutions offer researchers a powerful and well-established method for evaluating the efficacy of antiviral compounds.
Normal cells can absorb reactive dyes during metabolism, but when the cells are infected with a virus, they lose their ability to absorb the dye, resulting in the formation of colorless plaques. When a virus is neutralized by a specific antibody and then infects a cell, the number of cellular plaques formed decreases accordingly. Therefore, the titer of the antibody that neutralizes the virus can be calculated from the number of cellular plaques.
The Plaque Reduction Assay measures the efficiency with which the virus forms plaques in the presence of different concentrations of the assay. The time required for plaque visualization depends on the kinetics of viral replication and ranges from 24 hours to several weeks. Because plaques must be at least 1 mm wide to be accurately scored (especially by the naked eye), this analysis is typically performed on 24-well or 6-well plates, as any smaller wells may compromise readability and resolution.
The plaque reduction neutralization test (PRNT) is a variant of the plaque reduction assay and is considered the gold standard for detecting neutralizing antibodies to certain viruses, such as dengue virus. The basic design of the PRNT is to generate virus-antibody interactions in test tubes or microtiter plates and then apply the mixture to virus-sensitive cells to measure the effect of the antibody on virus infectivity.
PRNT is a versatile tool in virology with applications in diagnosing viral infections, characterizing toxins, and vaccine development. It can be used to detect antibodies in a patient's serum or identify viruses in diseased tissue, aiding in diagnosis. PRNT can also be utilized with antitoxin serum to identify and quantify bacterial toxins in pathological material. Furthermore, it is a valuable technique for determining the potency of antiviral serums and antitoxins, as well as identifying and classifying newly isolated viruses.
As a solution provider for virology and microbiology research, Creative Diagnostics offers comprehensive Plaque Reduction Assay services (e.g., PRNT) to empower researchers to accelerate breakthroughs in the field of virology. The company is dedicated to providing antiviral testing and customized early detection solutions to life science companies working in the field of antiviral and infectious disease diagnosis.
For viruses with only minor cellular lesions or viruses that take a long time to develop phagocytosis, Creative Diagnostics uses immunostaining of infected foci to provide faster results in a smaller format without sacrificing accuracy. The company also utilizes standard sera as a baseline for each result, allowing comparisons between laboratories and over time.
For more information about the Plaque Reduction Assay services, please visit https://antiviral.creative-diagnostics.com/plaque-reduction-assays.html.
About Creative Diagnostics
Headquartered in New York, Creative Diagnostics is a consulting and experimental service provider specializing in virology and microbiology. The company provides comprehensive solutions to conquer obstacles in virology and microbiology research, from high-security infrastructure provision, biosafety regulation elucidation, to expert viral system assistance.
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Creative Diagnostics, a leading manufacturer and supplier of antibodies, small molecule conjugates and critical assay reagents for food, feed, environmental and toxicological sample analysis, announced the launch of its comprehensive portfolio of Ochratoxin Test Reagents. These reagents are specifically designed to aid in the accurate detection of ochratoxin A (OTA) in food products.
OTA is a para-chlorophenolic mycotoxin produced by molds of the genera Aspergillus and Penicillium. It is the most abundant and relevant mycotoxin of this group, while ochratoxins B and C are of lesser importance. This mold toxin is extremely harmful to human and animal health. Dietary exposure to ochratoxins can cause acute nephrotoxicity in mammals. In addition to its apparent nephrotoxicity, ochratoxin A has hepatotoxic, teratogenic, carcinogenic and immunosuppressive properties.
The main sources of ochratoxin are cereals, including barley, rye, wheat, corn, sorghum and oats. It is also found in commodities such as coffee beans, dried fruits and grapes, including wine and raisins. In addition, meat and meat products may be contaminated with this toxin. Regulations on OTA limits vary from country to country. Typical limits are 5 µg/kg for unprocessed cereals, 2 µg/kg for wine and 0.5 µg/kg for infant formula.
Ochratoxin A is of particular concern because it may be a human carcinogen, making its detection especially important. Recognizing the importance of accurate and reliable testing methods to ensure the safety of food products, Creative Diagnostics now offers a wide range of reagent products to assist in the accurate detection of ochratoxin, including Ochratoxin Immunoaffinity Columns, Ochratoxin ELISA Kits, Anti-Ochratoxin Monoclonal Antibodies.
Based on advanced detection technologies, these new Ochratoxin Test Reagents enable accurate detection of ochratoxin levels in a range of food samples, including grains, feedstuff, and processed foods, providing high sensitivity and specificity. For example, the Ochratoxin A Colloidal Gold Kit, a lateral flow immunoassay, can be used to detect ochratoxin A in grain and feed.
The new Ochratoxin Test Reagents are designed to assist food safety professionals in meeting their strict quality control standards. They are suitable for use in various testing applications and allow food safety professionals to choose the most appropriate testing method based on their specific needs and requirements. Creative Diagnostics remains committed to providing cutting-edge solutions for food safety testing. The launch of these new Ochratoxin Test Reagents shows the company's dedication to ensuring the safety and quality of food products.
The new Ochratoxin Test Reagents from Creative Diagnostics offer a rapid, sensitive, and reliable solution for ochratoxin detection. For more information about Creative Diagnostics' food safety testing products, please visit https://www.creative-diagnostics.com/food-analysis/tag-ochratoxin-36.htm.
About Creative Diagnostics
Creative Diagnostics is a leading manufacturer and supplier of antibodies, small molecule conjugates and critical assay reagents for the analysis of food, feed, environmental and toxicological samples. Its product portfolio covers a wide range of analytes including mycotoxins, drug residues, pesticides, contaminants, food allergens, food pathogens and vitamins. The company also assists customers in the rapid development, manufacture and commercialization of small molecule antigens, antibodies and food safety lateral flow strips for any market segment.
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