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Gene expression techniques based on microarrays have been applied to high-throughput, large-scale RNA-level investigations, including the identification of genes with differential expression. Nevertheless, the hybridization-based nature of microarray technology has restricted their application. RNA-seq through cDNA sequencing has been made possible by developments in massively parallel DNA sequencing methods. Compared to conventional microarray technologies, RNA-seq is a highly advantageous inst...
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